光动力医学网新版测试。。。

通过抑制ABCG2来增强光动力治疗大肠癌的疗效

学科进展 pdtmed 752次浏览 0个评论

 导读:韩国加图立大学医学研究中心和波士顿哈弗医学院麻省总医院的学者们联合在BMC Cancer杂志(2015年7月7日版)发表名为“

Enhanced efficacy of photodynamic therapy by inhibiting ABCG2 in colon cancers”的研究论文,显示抑制ABCG2可能增强光动力治疗大肠癌的疗效。

具体摘要信息如下:

BACKGROUND:背景

Photodynamic therapy (PDT) contains a photosensitizing process, which includes cellular uptake of photosensitizer and delivery of light to the target.

光动力疗法包含了一个光敏化过程,其包括了肿瘤分子对光敏剂的摄取和传递特定的激光到靶部位。


ATP-binding cassette subfamily G2 (ABCG2) regulates endogenous protoporphyrin levels. In human colon cancers, it is not fully examined the role of ABCG2 in porphyrin-based photodynamic therapy.

ATP结合转运蛋白亚家族成员G2(ABCG2)调节内生性原卟啉的水平。在人类大肠癌中,还没有完全确认ABCG2在卟啉光动力疗法中的作用。


METHODS: 方法

SW480 and HT29 cells were selected because they showed low and high ABCG2 expression levels, respectively.

选择SW480 HT29 细胞,因为它们分别显示为ABCG2 表达低水平和高水平。


Pyropheophorbid-a (PPa) was used as a photosensitizer.Cells were exposed to a 670 nm diod laser. Cell viability and necrosi apoptosis was examined.Production level of singlet oxygen was detected with the photomultiplier-tube s/ -based singlet oxygen detection system.

PPa作为光敏剂,给予670nm激光照射。检测细胞的生存能力和坏死调亡情况。通过光电倍增管或基于其结构的单态氧检测系统检测单态氧的产生水平。


RESULTS:  结果

SW480 cells, which expressed lower level of ABCG2, showed the higher uptake of PPa than HT-29 cells. The uptake level of PPa was significantly correlated with the decreased cell viability after PDT.

表达ABCG2 水平更低的SW480细胞与HT-29细胞相比,显示摄取更高水平的PPa(光敏剂)。 PPa的摄取水平与PDT治疗之后细胞生存能力的减弱具有重大的相关性。


Pretreatment with a ABCG2 inhibitor, Ko-143, significantly enhanced the PDT efficacy in HT29 cells compared to vehicle-pretreated cells.

对于HT29细胞,在治疗之前给予ABCG2 抑制剂Ko-143,与媒介物预处理细胞相比,大大的增强了PDT的疗效。


To confirm the ABCG2 effect on PDT, we established ABCG2 over-expressing stable cells in SW480 cells (SW480/ABCG2). Furthermore, SW480/ABCG2 cells showed significantly decreased PDT effect compared to the control cells.

为确认ABCG2 PDT疗效的影响,我们在SW480细胞中制定了ABCG2超表达的稳定细胞(SW480/ABCG2)。而且SW480/ABCG2 细胞与对照组细胞相比显示,大大的降低了PDT的效果。


The increased or decreased cell survival was significantly correlated with the production level of singlet oxygen after PDT.

增加或降低细胞的生存能力与PDT之后单态氧生成水平直接相关。


CONCLUSION(结论):


ABCG2 plays an important role in determining the PDT efficacy by controlling the photosensitizer efflux rate. This implies the control of ABCG2 expression may be a potential solution to enhance photosensitivity.

通过控制光敏剂的外排率,ABCG2PDT的疗效具有重要的作用。这意味着,控制ABCG2的表达来增强光敏特性是一种可能的解决方案。

 

文章来源:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494642/


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